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| YOKOYAMA, Naoaki; UENO, Akio; MIZUNO, Daisuke; KUBOKI, Noritaka; KHUKHUU, Altangerel; IGARASHI, Ikuo; MIYAHARA, Tokuji; SHIRAISHI, Takashi; KUDO, Ryuta; OSHIRO, Mamoru; ZAKIMI, Satoshi; SUGIMOTO, Chihiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; 横山, 直明; 五十嵐, 郁男; 松本, 高太郎; 猪熊, 壽. |
| Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore, MPSP genotype-specific PCR methods were... |
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Palavras-chave: Cattle; Genotype-specific PCR; Japan; MPSP gene; Theileria orientalis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3584 |
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